Author(s): Towbin H, Staehelin T, Gordon J
A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. The immobilized proteins were detectable by immunological procedures. All additional binding capacity on the nitrocellulose was blocked with excess protein; then a specific antibody was bound and, finally, a second antibody directed against the first antibody. The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. In the latter case, as little as 100 pg of protein was clearly detectable. It is anticipated that the procedure will be applicable to analysis of a wide variety of proteins with specific reactions or ligands.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/388439
Author(s): Walz Y, Wegmann M, Dech S, Raso G, Utzinger J
Author(s): Tuhebwe D, Bagonza J, Kiracho EE, Yeka A, Elliott AM, et al.
Author(s): Hotez PJ, Bottazzi ME, Strych U, Chang LY, Lim YA, et al.
Author(s): Price A, Verma A, Welfare W
Author(s): Brunet J, Pfaff AW, Hansmann Y, Gregorowicz G, Pesson B, et al.
Author(s): Colley DG
Author(s): Omedo M, Ogutu M, Awiti A, Musuva R, Muchiri G, et al.
Author(s): Coustau C, Gourbal B, Duval D, Yoshino TP, Adema CM, et al.
Author(s): Ittiprasert W, Miller A, Myers J, Nene V, El-Sayed NM, et al.
Author(s): Ittiprasert W, Knight M
Author(s): Tamura Y, Torigoe T, Kukita K, Saito K, Okuya K, et al.
Author(s): Tamura Y, Torigoe T, Kutomi G, Hirata K, Sato N
Author(s): Ittiprasert W, Nene R, Miller A, Raghavan N, Lewis F, et al.
Author(s): Ghosh I, Eisinger SW, Raghavan N, Scott AL
Author(s): Raghavan N, Miller AN, Gardner M, FitzGerald PC, Kerlavage AR, et al.
Author(s): Raghavan N, Freedman DO, Fitzgerald PC, Unnasch TR, Ottesen EA, et al.
Author(s): Knight M, Ittiprasert W, Odoemelam EC, Adema CM, Miller A, et al.
Author(s): Lockyer AE, Emery AM, Kane RA, Walker AJ, Mayer CD, et al.
Author(s): Negrao-Correa D, Mattos AC, Pereira CA, Martins-Souza RL, Coelho PM
Author(s): Lockyer AE, Spinks JN, Walker AJ, Kane RA, Noble LR, et al.
Author(s): Porcelli D, Butlin RK, Gaston KJ, Joly D, Snook RR