Author(s): Zhang W, Chen L, Liu D
The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 μM HgCl2. SP1 was also highly resistant to other metals, including CdCl2, CoCl2, CrCl3, CuCl2, PbCl2, and ZnSO4, and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl2 and the removal of HgCl2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor, was around 5.0. LD50 of P. putida SP1 to flounder and turbot was 1.5 × 109 CFU. Biofilm developed by P. putida SP1 was 1- to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl2 contamination over a broad range of pH.
Referred From: https://link.springer.com/article/10.1007/s00253-011-3454-5
Author(s): Van DE, Baker A, Reeves AJM, Pollard RDS, Schat AJH
Author(s): Machado MD, Soares HM, Soares EV
Author(s): Rizwan M, Meunier JD, Davidian JC, Pokrovsky OS, Bovet N, et al.
Author(s): Yousaf B, Liu G, Wang R, Rehman MZ, Rizwan MS, et al.
Author(s): Ogar A, Sobczyk Å, Turnau K
Author(s): Yang YR, Liang Y, Ghosh A, Song YY, Chen H, et al.
Author(s): Seth CS
Author(s): Muleta D
Author(s): Buddrus SK, Schmid M, Schreiner K, Welzl G, Hartmann A, et al.
Author(s): Rolf AO, Bakken LR
Author(s): Moreno JL, Hernandez T, Garcia C
Author(s): Rizwan M, Ali S, Adrees M, Rizvi H, Rehman MZ, et al.
Author(s): Wang S, Wang F, Gao S
Author(s): Sayyed RZ, Jamadar DD, Patel PR
Author(s): Woolf D, Amonette JE, Street-Perrott FA, Lehmann J, Joseph S, et al.
Author(s): Abbas T, Rizwan M, Ali S, Adrees M, Rehman MZ, et al.
Author(s): Zheng R, Sun G, Li C, Reid BJ, Xie Z, et al.
Author(s): Warnock DD, Lehmann J, Kuyper TW, Rillig MC